Metabolic Flux Analysis: Methods and Protocols opens up the field of metabolic flux analysis to
those who want to start a new flux analysis project but are overwhelmed by the complexity of
the approach. Metabolic flux analysis emerged from the current limitation for the prediction of
metabolic fluxes from a measured inventory of the cell. Divided into convenient thematic parts
topics in this essential volume include the fundamental characteristics of the underlying
networks the application of quantitative metabolite data and thermodynamic principles to
constrain the solution space for flux balance analysis (FBA) the experimental toolbox to
conduct different types of flux analysis experiments the processing of data from 13C
experiments and three chapters that summarize some recent key findings. Written in the
successful Methods in Molecular Biology series format chapters include introductions to their
respective topics lists of the necessary materials and reagents step-by-step readily
reproducible protocols and notes on troubleshooting and avoiding known pitfalls.Authoritative
and easily accessible Metabolic Flux Analysis: Methods and Protocols presents protocols that
cover a range of relevant organisms currently used in the field providing a solid basis to
anybody interested in the field of metabolic flux analysis.
