This detailed new edition provides a comprehensive collection of protocols applicable to all
members of the Coronavirinae sub-family currently and that are also transferrable to other
fields of virology. Beginning with a section on detection discovery and evolution the volume
continues with coverage of propagation and titration of coronaviruses genome manipulation
study of virus-host interactions as well as imaging coronavirus infections. Written for the
highly successful Methods in Molecular Biology series chapters include introductions to their
respective topics lists of the necessary materials and reagents step-by-step readily
reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge Coronaviruses: Methods and Protocols Second Edition serves as
a valuable guide to researchers working to identify and control viruses with increased
potential to cross the species barrier and to develop the diagnostics vaccines and antiviral
therapeutics that are required to manage future outbreaks in both humans and animals.