EAN: 9781461413899

SPRINGERBRIEFS IN FOOD HEALTH AND NUTRITION H...

The detection of genetically modified organisms (GMOs) is becoming very complex  with new GMOs
approved and unapproved  constantly entering world markets. Traceability and labelling of GMOs
is defined in regulations worldwide  demanding accurate and reliable testing to support the
requirements of legislation. This Brief provides the current state-of-the-art on all key topics
involved in GMO testing and is a source of detailed practical information for laboratories.
Special focus is given to qualitative and quantitative real-time PCR analysis relevant to all
areas where detection and identification rely on nucleic acid-based methods. The following
topics  important for testing laboratories  are also discussed: organization of the laboratory
focusing on aspects of the quality system and methods for testing  validation and verification
of methods  and measurement uncertainty. The Brief also discusses the new challenges of GMOs
and novel modified organisms  using new technologies  and the possible solutions for GMO
detection  including bioinformatics tools. Finally  legislation on GMOs and sources of
information on GMOs are provided  which are relevant not only to testing laboratories  but to
anyone interested in GMOs. The authors of this Brief have many years of experience in GMO
testing  development of real-time PCR methods  implementation of quality system requirements 
validations and verification of methods  and measurement uncertainty. The National Institute of
Biology is a highly qualified research laboratory and a National Reference Laboratory  which
also performs routine analyses of food  feed and seed. The Institute for Health and Consumer
Protection of the European Union Joint Research Centre has extensive knowledge and experience
of GMO detection. It hosts the European Union Reference Laboratory for GM Food and Feed in
addition to chairing the European Network of GMO Laboratories.

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