Regulatory B Cells: Methods and Protocols present the current experimental set-ups and
methodologies useful for the identification and characterization of B cells with suppressive
functions and for the study of their biological and immunological properties. Organized into
four sections this detailed volume covers the basic methods for the isolation and
immunophenotypical characterization of specific B cell subsets from mouse and human tissues
methods for the investigation of the mechanisms of immune suppression operated by B cells
several experimental approaches for the ex vivo generation expansion of IL-10-producing B cells
as well as procedures for the study of the immune suppressive function of B cells in specific
pathological settings. Written in the highly successful Methods in Molecular Biology series
format chapters include introductions to their respective topics lists of the necessary
materials and reagents step-by-step readily reproducible laboratory protocols and tips on
troubleshooting and avoiding known pitfalls.Practical and authoritative Regulatory B Cells:
Methods and Protocols serves as an ideal guide for immunologists as well as for cell and
molecular biologists interested in the intricacies of B cell biology.
