Rapid changes and significant progress have been made in the Agrobacterium field such as
genetically transforming plants for both basic research purposes and agricultural development.
In Agrobacterium Protocols Third Edition Volumes 1 and 2 a team of leading experts and
veteran researchers describe in detail techniques for delivering DNA to plant cells and
permanently altering their genomes. This edition emphasizes agricultural crops and plant
species with economic values with updated protocols on 32 plant species and protocols
involving 19 new species. Together with the 1st and 2nd editions these two volumes offer
Agrobacterium-mediated genetic transformation protocols for a total of 76 plant species. For a
number of important plants such as rice barley wheat and citrus multiple protocols using
different starting plant materials for transformation are included. Volume 2 contains 29
chapters with updated techniques for industrial plants root plants nuts and fruits tropic
plants and other important plant species. Written in the highly successful Methods in
Molecular Biology series format chapters include introductions to their respective topics
lists of the necessary materials and reagents step-by-step readily reproducible laboratory
protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and
cutting-edge Agrobacterium Protocols Third Edition facilitates the transfer of this rapidly
developing technology to all researchers in both fundamental and applied biology.
