This volume presents 30 state-of-the-art protocols and reviews to set up and apply primary
hepatocyte cultures for research and screening purposes. The first part of the book focuses on
the use of these particular liver-based in vitro models to study the different aspects of the
hepatocyte life cycle including cell growth differentiation and cell death. The second part
of the book is targeted towards the demonstration of the applicability of primary hepatocyte
cultures or liver-based in vitro models derived thereof for functionality and toxicity
testing. Written in the highly successful Methods in Molecular Biology series format chapters
include introductions to their respective topics lists of the necessary materials and reagents
step-by-step and readily reproducible laboratory protocols and key tips on troubleshooting and
avoiding known pitfalls.Authoritative and practical Protocols In In-Vitro Hepatocyte Research
is intended for basic and applied researchers in the area of pharmacology and toxicology both
in academic and industrial settings.
