This volume explores the considerable efforts that have been directed towards the development
of G Protein-Coupled Receptors (GPCR) screening assays in order to disclose GPCR acting
compounds elucidate signaling mechanisms or evaluate compound's efficacy. New discoveries in
the field along with the widely recognized need for better and safer pharmaceutical drugs
constitute the main motivation for this book. Readers both beginners and experienced
researchers will receive an updated overview of not only the established but also the
innovative technologies that promise to advance GPCR drug research. This book is organized into
two major parts: the introductory part discusses the necessary foundations for the
understanding of GPCR action and the rationale behind the design of the available screening
assays and part two provides detailed protocols for different screening approaches. Written in
the highly successful Methods in Molecular Biology series format the chapters include the kind
of detailed description and implementation advice that is crucial for getting optimal results
in the laboratory.Practical and innovative G Protein-Coupled Receptor Screening Assays:
Methods and Protocols reaches out to everyone involved in the discovery of GPCR-active drugs
and provides a transversal overview of the different levels of GPCR signaling addressable in
the different screening strategies and presents practical examples of how current assay
technologies are contributing to new paradigms in GPCR drug research.
