This volume opens by covering two main types of approaches widely used to determine essential
genes: single-gene knockouts and transposon mutagenesis in both prokaryotes and Candida
albicans. Given the significant advancement in the computational predictions of microbial
essential genes the second half of the book examines four main types of approaches:
comparative genomics supervised machine learning constraint-based methods and corrections of
transposon mutagenesis data as well as databases and servers that are often used in studying
gene essentiality. Written in the highly successful Methods in Molecular Biology series format
chapters include an introduction to their respective topics lists of the necessary materials
and reagents step-by-step readily reproducible laboratory protocols and tips on
troubleshooting and avoiding known pitfalls.Authoritative and up-to-date Gene Essentiality:
Methods and Protocols will aid researchers who wish to further our knowledge in this vital
field of study.
