This detailed volume addresses recent developments in phosphoproteomic techniques with a
particular focus on the plant system. Over the recent decades proteomic methods were refined
to study the significance and dynamics of protein phosphorylation in various biological
contexts. However working with plant tissue imposes particular challenges to the biologist
which are attributed to the rigid cell wall making protein extraction more difficult the
skewed protein abundance with Rubisco as a highly abundant protein and a large central vacuole
leading to low protein yield and increased degradative enzyme activity. The methodologies in
this book seek to move beyond these issues. Written for the Methods in Molecular Biology series
chapters include introductions to their respective topics lists of the necessary materials and
reagents step-by-step readily reproducible laboratory protocols and tips on troubleshooting
and avoiding known pitfalls.Practical and authoritative Plant Phosphoproteomics: Methods and
Protocols serves as an ideal reference for researchers investigating this vital area of plant
science.
