This volume provides detailed molecular analysis of the associated pathways and cell types
involved in Celiac Disease and embraces new technologies such as next generation sequencing.
The book is divided into three parts: Part I gives a background overview with a number of
reviews to describe the history and nature of the disease its diagnosis the role of animal
models and study designs for investigating genetic susceptibility. Part II describes molecular
techniques including tissue culture isolation and cloning of relevant cell types high
content analysis of biopsies and HLA genotyping. The final part of the book begins with an
outline of scripting for data management focusing on tools that are freely available to
researchers who wish to explore them. It also describes analysis pipelines for bioinformatics
prediction of antigenicity quality control and analysis of GWAS data and transcriptome
analysis by next generation sequencing. Written in the highly successful Methods in Molecular
Biology series format chapters include introductions to their respective topics lists of the
necessary materials and reagents step-by-step readily reproducible laboratory protocols and
tips on troubleshooting and avoiding known pitfalls.Authoritative and thorough Celiac Disease:
Methods and Protocols brings together novel and more traditional methods in molecular biology
and immunology in order to provide a tool-kit for all stages for Celiac Disease research-from
the practicalities of identifying obtaining stabilizing and maintaining high quality samples
to molecular analysis and bioinformatics.
