This detailed volume focuses on methods for the characterization of aggregation processes that
lead to the formation of amyloid fibrils and amyloid oligomers which feature in the etiology of
a variety of human disorders collectively known as amyloidoses. The scope of the collection
includes techniques for visualizing early steps on the amyloid formation pathway methods for
capturing and characterizing oligomeric potentially toxic intermediates strategies for
preparing and characterizing mature amyloid fibrils and approaches for understanding templating
and transmission of amyloid aggregates. Written in the highly successful Methods in Molecular
Biology series format the chapters include introductions to their respective topics lists of
the necessary materials and reagents step-by-step readily reproducible laboratory protocols
and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical Protein
Amyloid Aggregation: Methods and Protocols serves as an ideal guide for biochemists and
biophysicists with an interest in elucidating the mechanisms of protein amyloid formation as
well as chemists pharmacologists and clinicians with an interest in leveraging an
understanding of such mechanisms for the purpose of therapeutic development.
