This volume presents an overview of recent advances innovative applications and future
prospects of in vitro embryogenesis in higher plants. The book's chapters are divided into five
parts: Part I contains reviews on general topics (microspore zygotic and somatic embryogenesis
in vitro and in vivo asexual embryogenesis advances on the genetic physiological and
proteomic knowledge of somatic embryo formation role of apoptosis and mitochondria in somatic
embryogenesis and innovation in the use of bioreactors). The remaining four parts discuss
step-wise protocols on somatic embryogenesis in selected horticultural plants (Part II) forest
trees (Part III) gametic embryogenesis (Part IV) and pivotal topics such as the detection of
epigenetics modifications during microspore embryogenesis the in vitro embryogenesis and plant
regenerations from isolated zygotes the synthetics seed production the induction and
maturation of somatic embryos and the cryostorage of embryogenic cultures (Part V). Written in
the highly successful Methods in Molecular Biology series format chapters include
introductions to their respective topics lists of the necessary materials and reagents
step-by-step readily reproducible laboratory protocols and tips on troubleshooting and
avoiding known pitfalls. Authoritative and thorough In Vitro Embryogenesis in Higher Plants
is a useful source of information and ideas for plant tissue culturists cell biologists
embryologists horticulturists and operators of commercial nurseries. This book will introduce
the fascinating work in in vitro embryogenesis in higher plants to students and young
scientists.
