This volume describes up-to-date techniques for improved production of secondary metabolites of
economic interest using field and laboratory methods. Biotechnology of Plant Secondary
Metabolism: Methods and Protocols explores different secondary metabolite classes whole-plant
and cell organ culture systems and environmental and genetic transformation-based modulation
of biochemical pathways. Special focus is given to cell and tissue specific metabolism
metabolite transport microRNA-based technology heterologous systems expression of enzymes and
pathways leading to products of interest as well as applications using both model and
non-model plant species. Written in the highly successful Methods in Molecular Biology series
format chapters include introductions to their respective topics lists of the necessary
materials and reagents step-by-step readily reproducible laboratory protocols and tips on
troubleshooting and avoiding known pitfalls. Practical and cutting-edge Biotechnology of Plant
Secondary Metabolism: Methods and Protocols is a great resource for scientists of
interdisciplinary fields--plant science plant physiology pharmacy molecular biology
biochemistry bioengineering and forestry--in reaching their goals of producing plant
biochemicals in a sustainable and efficient manner while minimizing impacts to the environment
and providing the required quantities of these commodities to industry.
