This volume provides a comprehensive collection of classical and cutting edge protocols and
techniques to examine the normal development and physiological functions of the
gastrointestinal system and to model the most common digestive diseases. The chapters focus on
diverse research topics including ex vivo systems to study gastrointestinal development and
functions in vivo imaging of the gastrointestinal tract isolation and characterization of
intestinal immune cells and animal models of gastrointestinal inflammation and cancer. The
Gastrointestinal Physiology and Diseases: Methods and Protocols book targets wide audience of
physiologists cell and developmental biologists immunologists and physician-scientists
working in the field of gastroenterology and beyond. Written in the highly successful Methods
in Molecular Biology series format chapters include introductions to their respective topics
lists of the necessary materials and reagents step-by-step readily reproducible laboratory
protocols and tips on troubleshooting and avoiding known pitfalls.Highly practical and clearly
written Gastrointestinal Physiology and Diseases: Methods and Protocols will serve both
seasoned researchers as well as newcomers to the field and will provide a unique resource and
expert guidance to modern laboratory techniques developed for examining normal functions and
diseases of the gastrointestinal tract.
