This volume discusses detailed protocols for the analysis of glycosylation at the level of free
glycans and glycopeptides. The book covers topics such as the importance of glycans in
eukaryotic life (with a focus on mammals and particularly humans) recent technologies that
allow the characterization of larger sets of samples and method repeatability and robustness
as well as higher throughout with respect to sample preparation measurement and data analysis.
The chapters spans a wide range of techniques including the analysis of fluorescently labeled
glycans with HPLC LC-MS analysis of glycopeptides both for glycosylation profiling and for
in-depth tandem mass spectrometric analysis of protein glycosylation and the analysis of
glycans with fluorescent labeling capillary electrophoresis in conjunction with laser induced
fluorescence detection. The chapters also detail specific samples types including brain tissues
N-glycans from in-vitro cell cultures milk oligosaccharides invertebrate and protest
N-glycans as well as plant glycans. Written in the highly successful Methods in Molecular
Biology series format chapters include introduction to their respective topics lists of the
necessary materials and reagents step-by-step readily reproducible laboratory protocols and
tips on troubleshooting and avoiding known pitfalls.Practical and thorough High-Throughout
Glycomics and Glycoproteomics: Methods and Protocols is an essential reference for researchers
planning to enter this rapidly evolving field.
