RNA Methylation: Methods and Protocols (Methods in Molecular Biology)

This volume provides a comprehensive collection of current methods and protocols to study posttranscriptional base modifications in RNA with special focus on methylation. The protocols in this book discuss state-of-the-art methods for investigating aspects of RNA methylation on different types of RNA. The protocols cover topics such as wet-lab techniques for the detection of methylation instructions for bioinformatics analyses of transcriptome-scale data and protocols for the functional examination of RNA modifications and enzymes. Written in the highly successful Methods in Molecular Biolog y series format chapters include introductions to their respective topics lists of the necessary materials and reagents step-by-step readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough RNA Methylation: Methods and Protocols is a valuable resource for biochemists and molecular biologists from various fields who wish to investigate different types of RNA methylations.