This detailed volume provides a toolbox for designing constructs tackling expression and
solubility issues handling membrane proteins and protein complexes and exploring innovative
engineering of E. coli. The topics are largely grouped under four parts: high-throughput
cloning expression screening and optimization of expression conditions protein production
and solubility enhancement case studies to produce challenging proteins and specific protein
families as well as applications of E. coli expression. Written for the highly successful
Methods in Molecular Biology series chapters include introductions to their respective topics
lists of the necessary materials and reagents step-by-step readily reproducible laboratory
protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical
Heterologous Gene Expression in E. coli: Methods and Protocols serves molecular biologists
biochemists and structural biologists those in the beginning of their research careers to
those in their prime to give both an historical and modern overview of the methods available
to express their genes of interest in this exceptional organism.
