This volume examines the structure and dynamics of the bacterial flagellum using bacterial
genetics molecular biology biochemistry structural biology biophysics cell biology and
molecular dynamics simulation. The chapters are divided into 4 parts: Part I describes
flagellar type III protein exports assembly and gene regulation in S. enterica Part II
explains how to isolate the flagella from the bacterial cell bodies and further explains how
to conduct high-resolution structural and functional analyses of the flagellar motor Part III
talks about how to measure flagellar motor rotation over a wide range of external load how to
measure ion motive force across the cytoplasmic membrane and how to measure dynamic properties
of the flagellar motor proteins by fluorescence microscopy with single molecule precision and
Part IV explores the structure and function of Spirochetal Vibrio Shewanella and
Magnetococcus flagellar motors. Written in the highly successful Methods in Molecular Biology
series format chapters include introductions to their respective topics lists of the
necessary materials and reagents step-by-step readily reproducible laboratory protocols and
tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive The
Bacterial Flagellum: Methods and Protocols aims to provide valuable and vital research to aid
in the investigation of the bacterial flagellum resulting from various bacterial species.
