This book presents multiple new and classical methods for studying the vital poly-ADP-ribose
(pADPr) pathway. Beginning with techniques for the detection and quantification of the product
of poly(ADP-ribose) polymerase (PARP) enzymatic activity and detection of variation in pADPr
production during the cell cycle the volume continues with sections on the identification of
pADPr protein acceptors methods focusing on studying molecular mechanisms of PARP functions in
eukaryotic cells particularly those involved in control of DNA repair and oxidative stress as
well as in expression regulation approaches to the in vitro reconstitution of PARP-1
interaction with chromatin the development and testing of small molecule PARP inhibitors and
the functions of understudied members of PARP family. Written for the highly successful Methods
in Molecular Biology series chapters include introductions to their respective topics lists
of the necessary materials and reagents step-by-step readily reproducible laboratory
protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical
Poly(ADP-Ribose) Polymerase: Methods and Protocols Second Edition serves as an ideal companion
to the first edition for scientists whose investigations involve this important pathway.The
chapter 'Identifying and Validating Tankyrase Binders and Substrates: A Candidate Approach' is
published open access under a CC BY 4.0 license.
