This volume looks at the evolution of the topo field over the last year and discusses how DNA
topoisomerases are directly and indirectly involved in numerous cellular processes. The
chapters in this book cover various topics such as type 1 topos with an evolutionary
perspective antimicrobial agents targeting topos experimental protocols related to DNA
topology including a basic technique to measure DNA supercoiling in bacteria protocols for in
vitro studies including assays to detect RNA topo activity topo inhibitors and a study of
plasmid-mediated quinolone resistance in bacteria. Written in the highly successful Methods in
Molecular Biology series format chapters include introductions to their respective topics
lists of the necessary materials and reagents step-by-step readily reproducible laboratory
protocols and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and
comprehensive DNA Topoisomerases: Methods and Protocols is intended for specialists and
non-specialists with an overview of selected hot topics in the field and the latest protocols
to study DNA topology topoisomerases functions and inhibition by various compounds.
