This third edition volume expands on the previous editions by providing a comprehensive update
on the available technologies required to successfully perform DNA methylation analysis. The
different technologies discussed in this book analyze the global DNA methylation contents
comprehensive analyses using various NGS based methods for genome-wide DNA methylation analysis
along with precise quantification of DNA methylation levels on single CpG positions. The
chapters in this book are divided into 7 parts: an introduction to the field along with tips on
study design and data analysis global DNA methylation levels genome-wide DNA methylation
analysis highly multiplexed target regions locus-specific DNA methylation analysis DNA
methylation analysis of specific biological samples and hydroxymethylation. Written in the
highly successful Methods in Molecular Biology series format chapters include introductions to
their respective topics lists of the necessary materials and reagents step-by-step readily
reproducible laboratory protocols and tips on troubleshooting and avoiding known
pitfalls.Cutting-edge and thorough DNA Methylation Protocols Third Edition is a valuable
resource for postdoctoral investigators and research scientists who work with different aspects
of genetics and cellular and molecular biology as well as clinicians who are involved in
diagnostics or treatment of diseases with epigenetic components.
