This volume covers the mechanisms of pRb inactivation detailing repressive mechanisms commonly
associated to cancer and representative of the experimentally relevant tests used in the
establishment of cancer diagnosis and prognosis. Chapters contain protocols and in-depth
discussions for commonly used experimental approaches to assess the status and function of
components of the pRb pathway including pRb itself in cell lines and biological
samples.Written in the highly successful Methods in Molecular Biology series format chapters
include introductions to their respective topics lists of the necessary materials and reagents
step-by-step readily reproducible laboratory protocols and tips on troubleshooting and
avoiding known pitfalls.Authoritative and practical The Retinoblastoma Protein aims to serve
as a guide to assist molecular cancer biologists in their search for understanding of the
molecular functions of this preeminent tumor suppressor.
