This volume presents an assortment of traditional and emerging experimental procedures relevant
to Schwann cell research. The chapters are divided into four parts. Part I contains protocols
for in vitro culture purification and characterization of primary Schwann cells from diverse
species and stages of nerve development. It also contains protocols to create cancer cell lines
and engineered Schwann cells from unconventional sources via chemical conversion induced
differentiation or genetic intervention. Parts II and III outline a wide range of methodologies
used to study Schwann cells within in vitro and in vivo systems relevant to the analysis of
peripheral nerve development cancer axon degeneration regeneration and myelination. Last but
not least part IV outlines protocols for Schwann cell production collection labeling and
transplantation in the injured peripheral nerve and spinal cord of experimental animals and
human subjects.Authoritative and practical Schwann Cells: Methods and Protocols aims to aid
both experienced and new investigators to make progress in their research endeavors involving
Schwann cells.
