This detailed volume explores the use of Sertoli cells as models in male reproductive biology
or as supporters for other cell types and it answers the need for standardized protocols for
their in vitro culture. Prepared for non-specialists as well as experienced researchers that
may be interested in a multidisciplinary approach to study these cells the book examines these
remarkable cells that present some unique features that go far beyond the physical and
nutritional support of spermatogenesis. Written for the highly successful Methods in Molecular
Biology series chapters include introductions to their respective topics lists of the
necessary materials and reagents step-by-step readily reproducible laboratory protocols and
tips on troubleshooting and avoiding known pitfalls. Authoritative and practical Sertoli
Cells: Methods and Protocols serves as a valuable resource for experienced researchers in
reproductive biology particularly andrologists but also for future investigators and young
students starting to study these extraordinary cells.
