This third edition volume expands on the previous editions with more detailed research on the
characterization of antibody antigen interactions between different users with different
requirements. The chapters in this book are divided into four parts: Part One looks at the
entire native antigen and covers traditional structural biology techniques such as nuclear
magnetic resonance and x-ray crystallography. Part Two talks about protein fragments derived
from antigens and discusses binding regions within antigen sequence using bacterial surface
display and ELISA for example. Part Three describes the use of surface plasmon resonance
spectroscopy and biolayer interferometry and Part Four highlights methods used to identify new
antigens and assess antibody cross-reactivity. Written in the highly successful Methods in
Molecular Biology series format chapters include introductions to their respective topics
lists of the necessary materials and reagents step-by-step readily reproducible laboratory
protocols and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge
Epitope Mapping Protocols Third Edition is a valuable resource for anyone interested in
furthering their research in this expanding field.
