This volume compiles methodologies used for detailed studies of histone variants from their
basic properties to their functional roles in chromatin and as vectors of epigenetic
information. Its four sections cover experimental approaches to probe the biochemistry of
histone variants and variant nucleosomes their dynamics throughout the cell cycle and at
specific genomic locations their functional impact on chromosome organization and genome
stability and their importance in development disease and evolution. Written in the highly
successful Methods in Molecular Biology series format chapters include lists of the necessary
materials and reagents step-by-step readily reproducible laboratory protocols illustrations
on the expected results and tips on troubleshooting and avoiding known pitfalls. This
anthology brings together contributions from many of the most highly recognized investigators
in this community. Cutting-edge and thorough Histone Variants: Methods and Protocols is a
valuable resource for anyone interested in exploring the experimental and collaborative
possibilities in this evolving field.
