This volume explores numerous techniques used to study the ubiquitin proteasome system. The
chapters in this book are organized into five parts and cover topics such as determining the
mechanisms of action for E2s E3s and DUB enzymes the latest advances to study the formation
of poly-ubiquitin chains as well as their linkage types the binding partners of proteins in
the UPS methods for structure determination by x-ray crystallography cryo electron microscopy
and SAXS screening assays to select for degrons or modulators of E3s and DUBs proteomics
approaches in the ubiquitin field and methods to study 26S proteasome function. Written in the
highly successful Methods in Molecular Biology series format chapters include introductions to
their respective topics lists of the necessary materials and reagents step-by-step readily
reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.¿
Thorough and authoritative The Ubiquitin Proteasome System: Methods and Protocols is a
valuable resource for both experienced and novice scientists who are interested in expanding
their knowledge in this field.
