This detailed volume includes protocols that represent the breadth of microbial metabolomics
approaches to both large-scale and small-scale experiments with intention of highlighting
techniques that can be used for applications ranging from environmental microbiology to human
disease. Utilizing mass spectrometry as their primary measurement tool the chapters explore
microbial metabolomics metabolism and microbial physiology metabolite sample preparation
current analytical techniques used to profile primary and secondary metabolites and lipids as
well as establishing data analysis workflows for targeted metabolomics untargeted metabolomics
analysis of metabolic fluxes and genome-scale models. Written for the highly successful
Methods in Molecular Biology series chapters include introduction to their respective topics
lists of the necessary materials and reagents step-by-step readily reproducible protocols and
tips on troubleshooting and avoiding known pitfalls.Authoritative and practical Microbial
Metabolomics: Methods and Protocols serves as an ideal reference for both novice and advanced
users and can be adapted to similar analytical platforms or customized to suit the needs of the
researcher.
