This second edition volume expands on the previous edition with descriptions of recent
developments in the field. The chapters in this book cover topics such as monoclonal antibodies
for the treatment of melanoma production and purification of human monoclonal antibodies
humanization and optimization of monoclonal antibodies rapid chimerization of monoclonal
antibodies epitope mapping via phage display from single gene libraries recombinant
antibodies made by combining phage and yeast display selections production of stabilized
antibody fragments in the E. coli bacterial cytoplasm and transfected mammalian cells and
analysis of CAR T cells. Written in the highly successful Methods in Molecular Biology series
format chapters include introductions to their respective topics lists of the necessary
materials and reagents step-by-step readily reproducible laboratory protocols and tips on
troubleshooting and avoiding known pitfalls. Unique and thorough Human Monoclonal Antibodies:
Methods and Protocols Second Edition is a valuable tool for novice and expert researchers
interested in learning more about this evolving field.
