This detailed volume describes a series of techniques that are essential for evaluating the
efficacy of new checkpoint blockade therapies as well as understanding the mechanisms behind
the therapies that have already been approved. Beginning with a section on describing the tumor
microenvironment and evaluating the immune system at a systemic level the book continues by
covering functional assays that provide answers to questions that may be raised after studying
the immune system and its responses to immunotherapies as well as the use of animal models in
this research. Written for the highly successful Methods in Molecular Biology series chapters
include introductions to their respective topics lists of the necessary materials and reagents
step-by-step readily reproducible laboratory protocols and tips on troubleshooting and
avoiding known pitfalls. Authoritative and practical Immune Checkpoint Blockade: Methods and
Protocols serves to aid researchers in furthering our understanding of checkpoint blockage as
well as the study of tumor immunology and the development of new immunotherapies.
