This volume provides detailed descriptions on cutting-edge experimental methods to assess the
multiple and complex interactions between T. cruzi and its host. Chapters detail a series of
methods and protocols ranging from rather classical biochemical genetic approaches to modern
powerful and high-throughput '-omics' technologies to tackle this issue as well as novel
techniques to improve treatment and clinical evaluation of Chagasic patients. Genome-wide
mining strategies aimed at identifying potential parasite antigens and vaccine candidates and
an overview on the challenges and milestones encountered during T. cruzi genome assembly are
also included. Written in the highly successful Methods in Molecular Biology series format
chapters include introductions to their respective topics lists of the necessary materials and
reagents step-by-step readily reproducible laboratory protocols and tips on troubleshooting
and avoiding known pitfalls. Authoritative and cutting-edge T. cruzi Infection: Methods and
Protocols aims to provide students researchers and clinicians who are interested in the study
of Chagas disease an indispensable source of information.
