This detailed volume explores the NADPH oxidase family of enzymes in human physiology and
genetic disease in which early discoveries represent prime examples of the finest
translational from bed to bench and back studies. Methods are included for testing assembly and
function of multicomponent oxidase complexes and for analyzing reactive oxygen species (ROS)
generation in different systems by various means while addressing pitfalls of ROS probes
currently being used as well as protocols on NADPH oxidase regulation and their function in
cells. Written in the highly successful Methods in Molecular Biology series format chapters
include introduction to their respective topics lists of the necessary materials and reagents
step-by-step readily reproducible laboratory protocols and tips on troubleshooting and
avoiding known pitfalls. Authoritative and cutting-edge NADPH Oxidases: Methods and Protocols
will aid researchers working with the NOX DUOX family in continuing and expanding upon their
vital research.
