The second edition volume expands on the previous edition with new and updated chapters on the
latest methods used for studying presentation of antigenic peptides produced in the standard
processing pathways for MHC class I and II molecules. The new chapters cover topics such as
biochemical and cellular approaches to study the impact of the endoplasmic reticulum
aminopeptidases techniques to monitor MHC class I synthesis and degradation approaches to
measure processing efficacy description of different assays measuring MHC recycling and
protocols to produce MHC class II tetramers. Written in the highly successful Methods in
Molecular Biology series format chapters include introductions to their respective topics
lists of the necessary materials and reagents step-by-step readily reproducible laboratory
protocols and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and
comprehensive Antigen Processing: Methods and Protocols Second Edition is a valuable tool for
both novice and expert researchers interested in studying antigen processing and venturing out
further into this evolving field.
