This volume addresses challenging new questions surrounding stem cell-based chimera research.
This book is organized into three parts: Part One provides readers with a summary of different
human donor cell types. The chapters in this section discuss ways to evaluate new types of
pluripotent stem cells the derivation of naïve and primed pluripotent stem cells from mouse
preimplantation embryos and the ethical and regulatory complexities of informed consent for
the procurement of somatic cells. Part Two discusses methods for generating chimeras. The
chapters here look at chick models and human-chick organizer grafts generating human-pig
interspecies chimeras and techniques for transplanting mouse neural stem cells into a mouse
disease model for stroke. Part Three concludes the book with a look at ongoing ethical
controversies and new scientific directions. Chapters in this part cover the ethics of crossing
the xenobarrier animal welfare experimentation with spermatogonial stem cells and cautious
approaches to human-monkey chimera studies to further understand complex human brain disorders.
Written in the highly successful Methods in Molecular Biology series format chapters include
introductions to their respective topics lists of the necessary materials and reagents
step-by-step readily reproducible laboratory protocols and tips on troubleshooting and
avoiding known pitfalls.Cutting-edge and thorough Chimera Research: Methods and Protocols is a
valuable resource for scientists interesting in using chimeras as a research tool while also
taking into consideration their complex ethical scopes.
