This volume looks at key methodologies that are commonly used across antibody drug conjugates
(ADCs) programs. The chapters in this book cover topics such as conjugations to endogenous
cysteine residues click chemistry conjugations antibody conjugations via glycosyl remodeling
analysis of ADCs by native mass spectrometry characterization of ADCs by capillary
electrophoresis LC MS methods for studying lysosomal ADC catabolism and determination of ADC
concentration by ligand-binding assays. Written in the highly successful Methods in Molecular
Biology series format chapters include introductions to their respective topics lists of the
necessary materials and reagents step-by-step readily reproducible laboratory protocols and
tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical Antibody-Drug
Conjugates: Methods and Protocols is a valuable resource that aims to lower the activation
barrier when undertaking a new discipline and provides a toolbox for the next generation of
ADC scientists.
