Since the discovery of the pharmacological and toxicological importance of inhibiting the
cyclooxygenase (COX) enzymes by non-steroidal anti-inflammatory drugs (NSAIDs) much research
has gone into the development of methods to study the biological functions of COX-1 and COX-2.
In Cyclooxygenases: Methods and Protocols experts and pioneers in the field present the most
up-to-date in vitro and in vivo techniques routinely used in COX research. The volume delves
into essential topics such as the purification cloning and expression of COX enzymes as well
as in vitro assays aimed at determining the inhibitory potency of drugs on COX-1 and COX-2
activities with chapters describing protocols used for the extraction and measurement of the
prostanoids. This volume also describes in vivo disease models used to study the roles of COX-1
and COX-2 in gastrointestinal injury inflammation and pain. As a book in the highly
successful Methods in Molecular Biology(TM) series the protocols chapters include brief
introductions to their respective topics lists of the necessary materials and reagents
step-by-step readily reproducible laboratory protocols and notes on troubleshooting and
avoiding known pitfalls. Authoritative and cutting-edge Cyclooxygenases: Methods and Protocols
serves as an indispensable tool for all scientists seeking the treatment of inflammation pain
fever and other harmful conditions.
