During the past decade as the data on gene sequences and expression patterns rapidly
accumulated cell-free protein synthesis technology has also experienced a revolution becoming
a powerful tool for the preparation of proteins for their functional and structural analysis.
In Cell-Free Protein Production: Methods and Protocols experts in the field contribute
detailed techniques the uses of which expand deep into the studies of biochemistry molecular
biology and biotechnology. Beginning briefly with basic methods and historical aspects the
book continues with thorough coverage of protein preparation methods the preparation of
proteins that are generally difficult to prepare in their functional forms applications of the
cell-free technologies to protein engineering as well as some methods that are expected to
constitute a part of future technologies. Written in the highly successful Methods in Molecular
Biology(TM) series format the chapters include introductions to their respective topics lists
of the necessary materials and reagents step-by-step readily reproducible laboratory
protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and
cutting-edge Cell-Free Protein Production: Methods and Protocols aims to help researchers
continue the growth of the vital exploration of cell-free sciences and technologies in order to
better understand the dynamic lives of cells.
