Since the conception of this acclaimed series of volumes examining neural tissue culture the
expansion of neuroscience has continued to produce vital discoveries that utilize tissue
culture methodologies. The expert contributors to the fourth edition of Protocols for Neural
Cell Culture refine existing protocols and present the emerging new techniques and culture
media formulations linked with the many advances in neuroscience. While highlighting updated
experimental procedures for many of the classical neural tissue culture preps the volume
addresses topics such as regenerative medicine and the methods to grow and expand embryonic and
adult neural stem cells. Essential techniques for the isolation expansion and
cryopreservation of neural tissue from mouse rat and human sources are covered as well as
methods to assess aspects of cell function (calcium imaging) and cell death. With its detailed
step-by-step laboratory protocols this volume provides the kind of detailed description and
implementation advice that is crucial for getting optimal results. Authoritative and
cutting-edge Protocols for Neural Cell Culture Fourth Edition continues the previous
editions' tradition of exceptional quality and thorough coverage in tissue culture protocols
for today's neuroscience in order to inspire researchers in the field to further explore this
imperative area of study.
