Due to their importance to cell biology DNA topology-altering enzymes collectively DNA
topoisomerases and gyrases have been studied thoroughly from a biochemical perspective yet
the expansion of research at the molecular and cellular levels continues to show a great deal
of promise. In DNA Topoisomerases: Methods and Protocols experts in the field present a
compendium of protocols that extends into the new areas of research that have been recently
uncovered in order to elucidate the methodology needed to study topoisomerases in the molecular
and cellular context. The volume examines subjects such as the analysis of DNA topologies and
the interaction of topoisomerases with DNA as tools to measure topoisomerase functions the
binding of topoisomerases to specific sites in the genomes of eukaryotes including origins of
DNA replication as well as exploration of perturbed topoisomerase function. As a volume in the
highly successful Methods in Molecular Biology(TM) series chapters include introductions to
their respective topics lists of the necessary materials and reagents step-by-step
laboratory protocols and notes on trouble-shooting and avoiding known pitfalls. Cutting-edge
and easy-to-use DNA Topoisomerases: Methods and Protocols provides key content which reflects
an expanded understanding of the functions of these essential enzymes in the hope of inspiring
researchers to further important discoveries in this dynamic field.
