Among the many types of DNA binding domains C2H2 zinc finger proteins (ZFPs) have proven to be
the most malleable for creating custom DNA-binding proteins. In Engineered Zinc Finger
Proteins: Methods and Protocols expert researchers from some of the most active laboratories
in this field present detailed methods guidance and perspectives. The volume contains
sections covering the engineering of ZFPs methods for the creation evaluation and delivery
of artificial transcription factors (ATFs) methods for the creation and evaluation of zinc
finger nucleases (ZFNs) and a collection of the several applications and assays beyond ATFs
and ZFNs including zinc finger transposases and ChIP-seq methodology amongst other subjects.
Written in the highly successful Methods in Molecular Biology(TM) series format chapters
include introductions to their respective topics lists of the necessary materials and reagents
step-by-step readily reproducible laboratory protocols and notes on troubleshooting and
avoiding known pitfalls. Comprehensive and cutting-edge Engineered Zinc Finger Proteins:
Methods and Protocols aims to aid both seasoned practitioners and new investigators with its
vital methods and insights as they seek to create the next generation of engineered ZFPs and
applications. TOC:The Generation of Zinc Finger Proteins by Modular Assembly.- Engineering
Single Cys2His2 Zinc Finger Domains using a Bacterial Cell-Based Two-Hybrid Selection System.-
Bipartite Selection of Zinc Fingers by Phage Display for Any 9-bp DNA Target Site.-
Structure-Based DNA Binding Prediction and Design.- Generation of Cell-Permeable Artificial
Zinc Finger Protein Variants.- Inhibition of Viral Transcription Using Designed Zinc Finger
Proteins.- Modulation of Gene Expression Using Zinc Finger Based Artificial Transcription
Factors.- Construction of Combinatorial Libraries that Encode Zinc Finger-Based Transcription
Factors.- Silencing of Gene Expression by Targeted DNA Methylation: Concepts and Approaches.-
Remodeling Genomes with Artificial Transcription Factors (ATFs).- Transgenic Mice Expressing an
Artificial Zinc Finger Regulator Targeting an Endogenous Gene.- Artificial Zinc Finger
Nucleases for DNA Cloning.- In Vitro Assessment of Zinc-Finger Nuclease Activity.- Assessing
Zinc Finger Nuclease-Associated Toxicity.- A Rapid and General Assay for Monitoring Endogenous
Gene Modification.- Engineered Zinc Finger Proteins for Manipulation of the Human Mitochondrial
Genome.- High Efficiency Gene Targeting in Drosophila with Zinc Finger Nucleases.- Using Zinc
Finger Nucleases for Efficient and Heritable Gene Disruption in Zebrafish.- A Transient Assay
for Monitoring Zinc Finger Nuclease Activity at Endogenous Plant Gene Targets.- Validation and
Expression of Zinc Finger Nucleases in Plant Cells.- Non-FokI-Based Zinc Finger Nucleases.-
Designing and Testing Chimeric Zinc Finger Transposases.- Seeing Genetic and Epigenetic
Information without DNA Denaturation Using Sequence Enabled Reassembly (SEER).- Zinc
Finger-Mediated Live Cell Imaging in Arabidopsis Roots.- Biophysical Analysis of the
Interaction of Toxic Metal Ions and Oxidants with the Zinc Finger Domain of XPA.- Preparation
and Zinc-Binding Properties of Multi-Fingered Zinc-Sensing Domains.- Using ChIP-seq Technology
to Identify Targets of Zinc Finger Transcription Factors.- Crystallization of Zinc Finger
Proteins Bound to DNABeyond DNA: Zinc Finger Domains as RNA-Binding Modules.
