With its complex and extensively regulated metabolism the study of the RNA lifecycle demands
tools that allow for the localization of RNAs to be observed either in an in situ setting or
preferably under in vivo conditions. In RNA Detection and Visualization: Methods and Protocols
the best and brightest investigators provide an up-to-date and in-depth description of basic
methods and protocols used for detecting and visualizing mRNAs in both fixed and live cells
from bacteria to mammals. For novices and experts alike this mix of classic in situ
hybridization and advanced live imaging techniques cell fractionation and affinity
purification procedures and bioinformatics tools gives researchers the most complete and
extensive array of research aids possible. As a volume written in the highly successful Methods
in Molecular Biology(TM) series format chapters include introductions to their respective
topics lists of the necessary materials and reagents step-by-step readily reproducible
laboratory protocols and expert tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge RNA Detection and Visualization: Methods and Protocols offers
well-honed techniques in order to inspire researchers around the world to further our knowledge
of the vital biological significance of RNA.
