SELDI is distinct from other TOF-MS technologies in that it couples features of chromatography
and mass spectrometry facilitating analyte enrichment and sample cleanup on an array surface.
In the growing field of proteomics SELDI technology has been widely used for biomarker
discovery and characterization in diverse applications including diagnostics drug development
and basic research. SELDI-based biomarker studies can typically be divided into four phases:
discovery validation purification and identification and assay development. SELDI-TOF Mass
Spectrometry: Methods and Protocols provides an overview of the current applications of
SELDI-TOF MS (surface enhanced laser desorption ionization time-of-flight mass spectrometry)
with an emphasis on study and experimental design data analysis and interpretation and assay
development. Written in the highly successful Methods in Molecular Biology(TM) series format
chapters contain introductions to their respective topics lists of the necessary materials and
reagents step-by-step readily reproducible laboratory protocols and notes on troubleshooting
and avoiding known pitfalls. Authoritative and accessible SELDI-TOF Mass Spectrometry: Methods
and Protocols will provide information on optimizing study design experimental protocols and
data analysis and interpretation to yield robust biomarkers and biomarker assays using
examples from different disease areas.
