As scientist begin to understand the complexity of lipid signaling and its roles in plant
biology there is an increasing interest in their analysis. Due to the low abundancy and
transient nature of some of these hydrophobic compounds this is not always easy. In Plant
Lipid Signaling Protocols expert researchers in the field detail experimental approaches by
which plant signaling lipids can be studied. These methods and techniques include analysis of
plant signaling lipids including detailed protocols to detect various relevant compounds by
targeted or non-targeted approaches to assay relevant enzyme activities in biological material
or using recombinant enzymes to test for specific binding of signaling lipids to protein
partners or to visualize signaling lipids or lipid-derived signals in living plant cells.
Written in the highly successful Methods in Molecular Biology series format chapters include
introductions to their respective topics lists of the necessary materials and reagents
step-by-step readily reproducible laboratory protocols and key tips on troubleshooting and
avoiding known pitfalls. Authoritative and practical Plant Lipid Signaling Protocols aids
plant researchers in the continuing to study the roles of lipid signals.
