Animal Cell Biotechnology: Methods and Protocols Third Edition constitutes a comprehensive
manual of state-of-the-art and new techniques for setting up mammalian cell lines for
production of biopharmaceuticals and for optimizing critical parameters for cell culture from
lab to final production. The volume is divided into five parts that reflect the processes
required for different stages of production. In Part I basic techniques for establishment of
production cell lines are addressed especially high-throughput synchronization insect cell
lines transient gene and protein expression DNA Profiling and Characterisation. Part II
addresses tools for process and medium optimization as well as microcarrier technology while
Part III covers monitoring of cell growth viability and apoptosis metabolic flux estimation
quenching methods as well as NMR-based techniques. Part IV details cultivation techniques and
Part V describes special applications including vaccine production baculovirus protein
expression chromatographic techniques for downstream as well as membrane techniques for virus
separation. Written in the successful Methods in Molecular Biology series format chapters
include introductions to their respective topics lists of the necessary materials and reagents
step-by-step readily reproducible protocols and notes on troubleshooting and avoiding known
pitfalls. Animal Cell Biotechnology: Methods and Protocols Third Edition provides a compendium
of techniques for scientists in industrial and research laboratories that use mammalian cells
for biotechnology purposes.
