Clear and concise this easy-to-use book offers an introductory course on the language of gene
cloning covering microbial plant and mammalian systems. It presents the nuts and bolts of
gene cloning in a well-organized and accessible manner. Part I of this book outlines the
essentials of biology and genetics relevant to the concept of gene cloning. Part II describes
common techniques and approaches of gene cloning ranging from the basic mechanics of DNA
manipulation vector systems process transformation to gene analysis. Part III & IV present
application technologies of major impact in agriculture biomedicine and related areas. The
ABCs of Gene Cloning Third Edition contains updates including a tutorial chapter on
gene-vector construction methodologies on exome sequencing in finding disease genes revised
topics on gene therapy and whole genome sequencing new developments for gene targeting and
genome editing as well as the current state of next generation sequencing. With more than 140
illustrations this new edition provides an invaluable text for students and anyone who have
interest in gaining proficiency in reading and speaking the language of gene cloning.
