This book is a practical review which focuses on computational analysis and on in silico
approaches towards the systematic discovery of various key functional gene expression elements
in microalgae as a model. So far in this regard very little information is available.
Efficient stepwise procedures for analysing the matrix attachment regions (MARs) are outlined
as well as for translation initiation sites (TIS) signal peptide (SP) sequences gene
optimization and transformation systems. These outlines can be efficiently deployed as
practical models for the systematic discovery of key expression elements and for the
optimization of cis transgenes in other micro organisms. The first chapter is an introduction
on the key gene expression elements analysed in this book including scaffold matrix attachment
regions translation initiation sites signal peptides as well as gene optimization. Chapter 2
focuses on systematic strategies and computational approaches toward in silico analysis of each
factor. The analyses outcomes is assessed individually in chapter 3 followed by developing the
specific conceptual models for each element in Chapter 4. The concluding remarks are discussed
in Chapter 5. This work is of interest to computational and experimental biologists interested
in transcriptional regulation analysis as well as to researchers and scientists who wish to
consider the use of bioinformatics and computational biology in design analysis or regulatory
reviews of key gene expression elements for the production of recombinant proteins experiments.
