An all-in-one practical guide on how to efficiently use chromatographic separation methods
Based on a training course that teaches the theoretical as well as practical aspects of protein
bioseparation to bioprocess professionals this fully updated and revised new edition offers
comprehensive coverage of continuous chromatography and provides readers with many relevant
examples from the biopharmaceutical industry. Divided into two large parts Protein
Chromatography: Process Development and Scale-Up Second Edition presents all the necessary
knowledge for effective process development in chromatographic bioseparation both on small and
large scale. The first part introduces chromatographic theory including process design
principles to enable the reader to rationalize the set-up of a bioseparation process. The
second part illustrates by way of case studies and sample protocols how the theory learned in
the first part may be applied to real-life problems. Chapters look at: Downstream Processing of
Biotechnology Products Chromatography Media Laboratory and Process Columns and Equipment
Adsorption Equilibrium Rate Processes and Dynamics of Chromatography Columns. The book closes
with chapters on: Effects of Dispersion and Rate Processes on Column Performance Gradient
Elution Chromatography and Chromatographic Column Design and Optimization. -Presents the most
pertinent examples from the biopharmaceutical industry including monoclonal antibodies
-Provides an overview of the field along with design tools and examples illustrating the
advantages of continuous processing in biopharmaceutical productions -Focuses on process
development and large-scale bioseparation tasks making it an ideal guide for the professional
bioengineer in the biotech and pharma industries -Offers field-tested information based on
decades of training courses for biotech and chemical engineers in Europe and the U.S. Protein
Chromatography: Process Development and Scale-Up Second Edition will appeal to
biotechnologists analytical chemists chromatographers chemical engineers pharmaceutical
industry biotechnological industry and biochemists.
