Most terrestrial plants live in symbiosis with arbuscular mycorrhizal fungi. In this study a
mycorrhizal-induced gene family of Medicago truncatula encoding putative Kunitz protease
inhibitors was functionally characterized by means of biomolecular biochemical microscopical
and in silico methods. Their putative target proteases were identified among a clan of serine
carboxypeptidases. Results suggest that both protein families work functionally together to
control mycorrhizal establishment.
