Beschreibung The recent rise of bio-succinic acid as an industrial platform chemical has
brought the rumen bacterium Basfia succiniciproducens a natural producer of this organic acid
into the focus of research. This work investigated the potential of B. succiniciproducens for
the production of carbon-three chemicals from sugars. Beside the optimization of improved
genetic tools the C3 chemicals alanine ¿-alanine and 3-hydroxypropionate (3-HP) were
targeted. A new alanine producer B. succiniciproducens ALA-1 exhibited good performance in
the bioprocess. The next step demonstrated the proof-of-concept for ¿-alanine synthesis using
the heterologous expression of aspartate 1 decarboxylase genes from Corynebacterium glutamicum
or Vibrio natriegens. The expression of two additional genes enabled the de novo synthesis of
up to 100 mg L-1 3-HP from glucose.
