This open access volume gathers a variety of models delivery systems and approaches that can
be used to assess RNA technology for exploiting antisense as a therapeutic intervention.
Beginning with a section on the design of antisense technology and their delivery the book
continues by covering model systems developed to evaluate efficacy both in vivo and in vitro
as well as methods to evaluate preclinically the toxicity associated with these new potential
drugs and intellectual property considerations. Written for the highly successful Methods in
Molecular Biology series chapters include introductions to their respective topics lists of
the necessary materials and reagents step-by-step readily reproducible laboratory protocols
and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical Antisense
RNA Design Delivery and Analysis provides basic knowledge and a large collection of methods
to facilitate the work of newcomers to this vibrant and expanding field. This book was
conceived thanks to the network DARTER (Delivery of Antisense RNA Therapeutics). DARTER is
funded by the EU Cooperation of Science and Technology (COST) which aims to enhance
interaction and collaborations between researchers in Europe and other countries.
